3.1. Results Obtained from Activating Samples of Alkaline and Acidic Water
Activating a sample of alkaline water for 15 minutes will change pH from 7.69 to 7.48, which means we are confronting the reduction in alkaline magnitude.
If the duration is 30 minutes, we will observe more reduction in alkaline magnitude representing the change of pH from 7.65 to 7.25. It is important to remember that activating the acidic water sample for 30 minutes will increase the pH from 5.73 to 6.89, which means that the acidic property or magnitude undergoes a considerable reduction.
What is important in this experiment is the fact that through the process of activation, reaching the pH to a balance point is accompanied by the reduction of acidic or alkaline properties. These results are obtained because of the existence of free radicals like H
+ and OH - in water that is activated in water structure and contains stronger hydrogen bonds compared to just the regular water. The amount of Ca and Mg in water that is activated for 30 minutes will reduce 72% and 18%, respectively. As a result, the hardness of water (Ca and Mg compounds) will reduce up to 45%.
According to these experiments, we noticed a considerable amount of alterations and important reductions in free radicals. This reduction is due to the connection of these free radicals, i.e. Ca
2+ and Mg 2+, to the activated water structure with stronger hydrogen bonds ( 3, 12, 13).
Furthermore, the movement and transmission of water, which is activated for 30 minutes, is increased 3% and its darkness increases as much as 18%.
These results show that the activation process has induced stronger molecular structures.
The free radicals that increase the molecular structure of the activated water and formation of the deposits which cause more darkness in water are mutually connected.
We also observed a reduction of 44% in bacteria counts after 15 minutes activation of lake water and as a result, the destruction and elimination of microscopic creatures in activated water, which proved the positive effects of sterilized activated water process (
Table 1. Activity and Infection
Association Natural Anolyte Infection Effective cause Active chlorine, oxygen compound (hypo chlorous acidazen - peroxide) 1/4 Ammonium antibacterial solution Activity, % 2 to 0.06 50 Being poisonous Minimum (Level 4) Level 3 Applicable areas Disinfection - cleaning and sterilizing the medical equipment General sterilizing Form and shape Colorless material with little oxidant odor Clear liquid with special little odor Dormant life More than 3 days preserving the destructive behavior Registration Index 95 - 0024 Index 96 - 0025 Factory owner Research and production of “Ogran” community Israeli company Y.Y.SL 3.2. Biological Effects of Activated Water and Its Sickness Treatment Property
Several experiments were performed on the lymphatic cells by Dr. John Stell in biotechnological laboratory where the transformed cells were polluted by the activated water and some plant complements for the duration of 24 hours.
These experiments continuously showed that the activated water stopped 33% of the metabolism of cancer cells. Also, it was capable of stopping 50% of the metabolism in dogs and 10% in the case of cats. The results gained for cancer cells of the human and dog were shown by the expression of P > 0.99.
The amount of cancer cells remaining in other groups was 100%.
This experiment showed the none-poisonous nature of cancer cells compared to their treatment by chemicals. It showed that within the same time duration, it halted the metabolism of normal cells, and the general comparison of these two was related to tumors and normal cells longevity when the chemical treatment was lower than 5%.
Another experiment was designed for the plants to compare a 15-day period of two groups of oil seeds (20 from each group) which were treated by normal and activated water.
13 germs with the length of 9 cm were created. The control group that was treated with regular water had 7 germs with the length of 4 cm each, which again showed the accelerated growth of plants being watered with activated water (
8). 3.3. Important Difference in Water Activation Process and Its Mechanisms
In a situation that the water molecule is divided and analyzed to its oxygen and hydrogen constitutes, its structure will be altered and its active properties will be preserved for many hours. The process for the activation of these kinds of molecules will be accelerated and they will be activated. In general, this process is called electrochemical activation. In cathode, several chemical compounds are regenerated with chemical reactions (the best known is OH-); hydroxides (ECA) as well as unsolvable or rarely solvable heavy metals are also formed.
Heavy metals (e.g. Hg, Cr, Pb, Ag, Au-Gold, Tu-Tungsten) have high atomic mass.
They exist in water in a very low concentrations but they are very poisonous (
The poisonous activities of heavy metals and other metals with lower weights in their ionized form are very obvious.
The above metals are shown with the expression of me exp (n
+), in which n represents the number of positive charges; the result is the regeneration of cathode ionized poisonous metals to hydroxides (E Me (OH)n). Metal hydroxides are chemically neutral and are not dangerous for human beings.
If they enter into stomach juice along with water, the acid in juice is not able to ionize them; for this reason, the Emerald Equipment decomposes the poisonous metals in the form of ions, but it is not capable of eliminating them (
10, 11). 3.4. Researches on Electrochemical Water
The diversity of main parameters for sea water and other new active parameters as well as pH and EH of both anolyte and catholyte and their combination has been studied along with solidification and evaporation.
The sea water activated bacteria (Anole) were also studied considering the pure tissues of the microorganisms with their special importance for sterilized hygien.
The outcomes of continuous fish breeding process with anode initiated particles, then those related to cathode from activated sea water indicated that the bacterial effect is performed under anole examination and this is when undesirable oxidation processes are hidden by cathode particle examination. This examination acts like anti-oxidation process and restricts the agent for oxidation interactions which release the radicals.
The new strategy called chitin (Poly-N-Steel-D-Glucose Amine) which is generated from chitin that contains the raw material (Sea Alga, Hammarus, Shrimp Shells, Crabs and the rest of Crustacea), is vastly popular. The raw protein material is inside the cathode electrolysis container which is demineralized inside the box (
7). 3.5. Nanosecond Evacuation (1/1 Billion Second) Inside Water is Effective for Disinfection
The water disinfection is a simple and effective process for power consumption at KW hour/liter and for this purpose, the number of 5 × 10 exp (-5) L exp (-) 10 exp (-4) counts of special bacteria (
E. coli) equivalent to per Liter CFU up to 5 × 7 Log is considered.
In the past, electro physical approaches were used for water disinfection which depened on the electricity consumption and mechanical energy. They were high strength platforms for producing the light waves of UV, which contained invisible rays of light inside the UV spectrum that scattered UV lamp radio lights and caused the separation of base material from new chemical material in an environment where there existed no electrical current. At the time of separation of these materials, some sound waves were produced which had an essential role in water disinfection. Normally, many of physical, chemical and biological effects of evacuation inside the water are not distinguished and are unclear. Moreover, the simple applied evacuation systems as well as power and accommodation of produced units are not clarified yet (
In the current reports, the explanations for a simple evacuation with high effectiveness are provided in a way that a special design for the electrodes with high nanosecond (1/1 billion second) voltage is used that is poured in water and accompanied by high energy of “E ≤ 0.35 J”.
The electrical evacuation depends on short time high voltage consumptions, low energy strokes, which is a very sophisticated duty for the electrodes.
We came to the conclusion that water disinfection using
E. coli had been destructive enough.
The evacuation was considered through the operation of voltage strokes for two heavy electrodes with even surfaces. The amino electrodes distance which is filled with small gas bubbles in water is also considered.
The water is quaked with water rings with the speed of 5 cm/s. The connecting strength of standard preserving units is as following:
(The duration of stroke t ≤ 100 seconds) - (The repetition speed of the stroke F = 10 Hz) - (Energy stroke of E ≤ 0.35 J)
In each stroke with the voltage of 40 KW, the evacuation has a narrow shape which can also be called “String”. This narrow channel relates the distance between evacuation and the exit (from one stroke to another in the opposite direction of the electrode surface). This last situation to some extent increases the stability for periodical property of the electrodes. Compared to large usage of needle with an even shape, the application of
E. coli and its results were heavily controlled, examined and were experimented on the filled medium size nutrients in a separate container. After 18 - 24 hours of being covered and exposed in the thermostat in the temperature of 37°C, the number of E. coli was counted. The number of adjacent cells under the experiment after the release of the energy was diminished ( 14- 16). 3.6. Limitations of Analyzed Live Cells in Electrochemical Activities (ECA)
In similar systems of pH and ORP (getting combined by the oxygen, diminishing the strength), there exists a comparison base for the analyzed live cells (related to animals, plants and bacteria) in the fields currently used. The unnatural compounds of pH and ORP are obtained with the help of the electrochemical activities which occur in active regions. The spread of microbe elimination activities using active chlorine on the material cells in different circumstances has been studied.
Some issues related to different or similar values regarding pH and ORP comparable with the activities of live analyzed cells (animal sperms) and the strength of male sexual cells and the bacteria cells has been examined, which emphasize the connections between them.
There are some factors which are effective in pH and ORP. The pH and ORP fields are only for adding the protective organic acid compounds and sodium bicarbonate or cathode activities (Negative Pole) which are being used.
According to the reports published, the erythrocytes that are analyzed inside many mammals’ blood are sensitive to hypochloride and this sensitivity is within their own dormant period.
Regarding the oglena plant cells living activities, the corresponding cells will deform and alter in response to fluctuations (going up and down) in the ORP amount.
The parametric composition of pH and ORP with coordination of electrode references in active anole, which was accessible from the beginning being also used in STEL plans, bears noticeable characteristics. Like anole processes, these devices have washing abilities for eliminating organic dirt coagulations (
9, 14, 17).
Through combination with oxygen, the sterilization activities are decreased but regarding the organic dirt, these products will abolish the effect of disinfection after spending enough time.
The plans for active electrochemical solutions are different from the still analogues and are important because of business problems as well as the plan for special thoughts controlling micro fluoride in human and animal life cycle.
The most common issue is that the Russian market is filled with antibacterial advertisements which are attached on the back of unessential household products. For this reason, the comparative information considering the special facts about Russian chemicals are far from scientific, experimental, and business aspects of the case.
The specifications for synthesized anole neutral activities are stated in STEL disinfection plans. The neutral anole is a sterilizer that creates a wide affected area which indicates the washing composition of the materials and their antibacterial effects get rid of all forms of known microbes (forms like viruses and spores). This substance is also used as a washing sterilizer. The saturated anole, through utilizing the peroxide particles, kills a large amount of microbacterial cells and disinfects them in a medical endoscope process in a very effective and quick manner (
In the vast and healthy living environments, anole environment is produced, because its effective disinfecting washer is not found within the mentioned environment yet.
3.7. Properties for Neutral Anolyte Bacteria of ANK Kind Relating to Hygienic Micro Organisms Industry and Fishing Industry
In the fishing industry, one of the most important issues is searching for the right disinfecting agents which are less poisonous, with minimal cause for sensitivity and allergy and easily movable on the applied surfaces. The boundary for preparation of new permissible disinfecting materials for food industry is extremely limited.
Recently, only some strands in the national economy are being used and are named disinfecting agents.
These solutions which are activated chemicals, contain active chlorine and free radicals with the name of sample neutral cathode and Anole, ANK.
As ANK is not widely used in fishing industry yet, the purpose for this study is:
1- The bacterial experiment of ANK for the existence of defined hygienic bacteria inside pure tissues and having the albumin charge which are both impurities of albumin properties - (blood, the remains of albumin fish, jelly material, etc.)
2- The description of small active chlorine in ANK which induces the effects of some bacteria on the hygienic well-known bacteria.
The abilities for anole bacteria in four well-known bacteria (the Huygens) were studied. The below chains called micro organism examiner are used:
Staphylococcus aureus 906, Enterobacter Cloaceae
Pseudomonas fluorescens, Bacillus cereus BKM 687
The 3-day bacteria cultures are used in this experiment which were multiplied on the FDA.
5 milliliters of fish stew water (FEB) were injected inside a U-shape tube for 72 hours at the temperature of 37°C.
S. aureus, E. lbaceae, Bacillus cereus, and P. fluorescens were placed in the thermostat. Like the experimental surfaces, materials are widely used in Fish breed processing industry such as sand planes - plastic mobile sheets - alloyed plastic, and steel ( 1, 18).
9 mL of salt solution was added to 1 mL of fish stew culture after 3 days.
2 mL of this suspension was used in a 7.5 × 7.5 cm cube.
Without any temperature, the container was dried out for 2 hours. The examination of the surface was performed with distributing the Anole ANK in 2 mL portions per cubic meter.
After the multiplications at the room temperature for 40 minutes, the damp area of the examined surface was dried with 2 strips of cotton.
The strips were placed inside 20 mL of salt solution containing 1 mL of hyposulfite 0.1 N and mixed completely for 1 - 2 minutes (
Then, the reduction happened and was injected over the surface of FPA. In experiments with organic charge instead of the salted solution, 9 mL of FPA was added to 1 mL of bacterial suspension.
The performance of bacterial reactions ANK as compared to the quantity of the multipliable cells in the controllable experiment was measured. The assumption was that the positive effect of the experiment should not have been less than 2 Logarithm of the reduction in multipliable cells with respect to the control.
With regards to the general form of the bacteria, gene factors “
Enterobacter” are mostly found in fish products. These factors are not only the main cause of animal waste liquids, but also carry some of fish diseases like Piscine ( 7).
For this reason, with the application of the bacteria of ANK, the chain
Enterobacter cloaceae is separated from sea products. The resulted actions of the ANK bacteria in a 3-day culture of Enterobacter cloaceae are shown in Table 2.
Table 2. Action Results for the ANK Bacteria Related to the
E. cloacae Culture on the Stone and Sandy Plane After 40 Minutes of Contact
Number of Cells That Can Multiply, CFU/mL Stone Material ANK, CL, Mg/l After the examination is washed Washed without examination Used from the sample future wash 4.2 × 10 2 No Protein - 2.6 × 10 2 3.5 × 10 6 The Stone Plane - Sandy 250 1.6 × 10 3 Protein- 2.5 × 10 3
Table 3 indicates that the number of multipliable E. cloaceae is clearly sloped (up to 3log10) in the application step and when drying over the experimented material (stony sandy plane).
Table 3. The Effectiveness of the Action of ANK Bacteria Related to the
P. fluarescens Over the Surface of Stone-Sand After 40 Minutes Contact
Number of Cells That Can Multiply, CFU/mL Stone Material ANK, CL-,Mg/l After the examination is washed Washed without examination Used from the sample future wash < 5 No Protein - 2.4 × 10 6 3.2 × 10 8 The Stone Plane - Sandy 350
The ANK bacteria without organic charge, which indicates the decreasing number of cells of 2log10, have very effective multiplication property (
In the existence of organic charge, the effect of ANK bacteria (active chlorine density is 250 mg/L) is ignorable and its amount is 35% of the initial count of bacteria.
Therefore, we can conclude that the planted surfaces with E. CBG (the
E. coli bacteria group) 250 mg/L ANK and the higher density of active chlorine are effective in succeeding the sterilized stone and sandy planes.
The gene agents/factors of
Psedomonos are generally in water, on fish and its products.
The vastest kinds of these genes are
Pseudomonas Fluorescens actually (P.F) in the study of the ANK bacteria activities ( 19).
The findings of this study for the evaluation of the effect of ANK over a 3-day culture of
P. fluorescens adjacent to the albumin impurities which are indicated in chart 2 are gathered together.
Table 3 shows in the applied process of drying over the stone planes, the amount of 2log10 reduction in the number of multipliable cells in the bacteria suspension has been seen.
The effect of ANK bacteria (350 Cl- mg/L) is noticeable and is added to the 6log10 reduction of initial bacteria count (
Overall, we can conclude that in a case of
P. flourescens and existence of albumin impurities, ANK with a density of 350 CL- mg/L can be used for sterilizing stone-sandy plate surfaces.
Staphylococcus with the title of experiment Staphylococcus aureus with 906 chains is very credible and recommended in evaluation of sterilizers’ effectiveness.
The effect of ANK action against
S. aureus with 906 chains over several surfaces is indicated in Table 3. 3.8. Sand Culture 72 Hours at 30°C
The given parameters indicate that the quantity of multipliable cells
S. aureus bacteria suspension diminishes as soon as using the culture over the experimented material and the surface becomes dry over the sandy-stony plate which is bilateral, over the plastic up to 2log10 CFU/mL, over Steel Alloy up to 3 - 1 10 log10/mL.