Assessment of Bactaslyde media in diagnosis of Salmonella in water and foods in comparison with standard method in order to use in crisis

AUTHORS

Hooman Motlagh 1 , * , Hamidreza Tavakoli 2 , Arasb Dabbagh Moghaddam 3 , Narjes Cheraghi 4 , Hesameddin Akbarein 5

1 Instructor, Department of Preventive Medicine, Golestan Hospital, AJA University of Medical Sciences, Tehran, Iran., Andorra

2 Assistant Professor, Department of Nutrition and Food Hygiene, Baghiatollah University of Medical Sciences, Tehran, Iran, Andorra

3 Instructor, Department of Social Medicine and Public Health, AJA University of Medical Sciences, Tehran, Iran, Andorra

4 Researcher, Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, Andorra

5 Researcher, Division of Epidemiology, Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, Andorra

How to Cite: Motlagh H, Tavakoli H , Dabbagh Moghaddam A , Cheraghi N , Akbarein H . Assessment of Bactaslyde media in diagnosis of Salmonella in water and foods in comparison with standard method in order to use in crisis, Ann Mil Health Sci Res. 2013 ; 11(2):e66215.

ARTICLE INFORMATION

Annals of Military and Health Sciences Research: 11 (2); e66215
Published Online: May 06, 2013
Article Type: Original Article
Received: February 19, 2013
Accepted: April 06, 2013

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Abstract

Background: Salmonellas are responsible for more than 40 percent of foodborne diseases in the world. Today, rapid diagnostic methods are essential for detecting these organisms in food and water especially in crisis. The aim of this study was to assess the Bactaslyde media usability in detection of salmonella and compare it with standard culture method.

Materials and Methods: In this laboratory study 20 samples of water and foods (pasteurized milk, water, raw Kabab and Raw Poultry Meat) were contaminated with salmonella standard strain (PTCC 1609) and then this salmonellas were detected by Bactaslyde culture media and standard culture method. Sensitivity, specificity, positive predictive value, negative predictive value and speed of two methods compared with each other.

Results: This study showed that Bactaslyde culture pouch number 4 can detect Salmonella in all 20 contaminated samples of food and water. The detecting time was 15 hours and 55 minutes in compare with 93 hours for standard method. Sensitivity of the test was 100%, specificity 90%, positive predictive value 90.91%, negative predictive value, 100% and validity of the test was 95%.

Conclusion: It was found that Bactaslyde culture method can detect Salmonella as a rapid test in all food and water samples and it can be recommended for using in crisis. More research with more samples and different foods is recommended.

Keywords

Water Diagnostic Test Kits Food Salmonella

© 2013, Annals of Military and Health Sciences Research. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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