Annals of Military and Health Sciences Research

Published by: Kowsar

Synergistic Effect and Antibacterial Activities of Extracts of Salvia and Rosemary Officinalis Against Escherichia coli Isolated from Clinical Urinary Tract Infection

Forouzandeh Amirian 1 , Nadia Kazemi Pour 1 , Sayed Mohammad Reza Khoshroo 1 , Ahmadreza Sayadi 2 , Afsaneh Karmostaji 3 and Seyed Mohsen Mousavi 4 , 5 , *
Authors Information
1 Department of Microbiology, Islamic Azad University, Kerman Branch, Kerman, Iran
2 Department.of Psychiatric Nursing and Mental Health and Social Determinants of Health Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
3 Infectious and Tropical Disease Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
4 Immunology of Infectious Diseases Research Center , Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
5 Department.of Laboratory Medicine, Faculty of Paramedical, Rafsanjan University Medical Sciences, Rafsanjan, Iran
Article information
  • Annals of Military and Health Sciences Research: December 2017, 15 (4); e80148
  • Published Online: December 8, 2017
  • Article Type: Research Article
  • Received: October 3, 2017
  • Revised: November 5, 2017
  • Accepted: December 1, 2017
  • DOI: 10.5812/amh.80148

To Cite: Amirian F, Kazemi Pour N, Khoshroo S M R, Sayadi A, Karmostaji A, et al. Synergistic Effect and Antibacterial Activities of Extracts of Salvia and Rosemary Officinalis Against Escherichia coli Isolated from Clinical Urinary Tract Infection, Ann Mil Health Sci Res. 2017 ; 15(4):e80148. doi: 10.5812/amh.80148.

Copyright © 2017, Annals of Military and Health Sciences Research. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Methods
3. Results
4. Discussion
  • 1. Mendoza-Palomar N, Balasch-Carulla M, Gonzalez-Di Lauro S, Cespedes MC, Andreu A, Frick MA, et al. Escherichia coli early-onset sepsis: trends over two decades. Eur J Pediatr. 2017;176(9):1227-34. doi: 10.1007/s00431-017-2975-z. [PubMed: 28770413].
  • 2. Mellata M, Johnson JR, Curtiss R 3rd. Escherichia coli isolates from commercial chicken meat and eggs cause sepsis, meningitis and urinary tract infection in rodent models of human infections. Zoonoses Public Health. 2018;65(1):103-13. doi: 10.1111/zph.12376. [PubMed: 28703468].
  • 3. Bouzada MLM, Fabri RL, Nogueira M, Konno TUP, Duarte GG, Scio E. Antibacterial, cytotoxic and phytochemical screening of some traditional medicinal plants in Brazil. Pharm Biol. 2009;47(1):44-52. doi: 10.1080/13880200802411771.
  • 4. Rosenbaum CD, Carreiro SP, Babu KM. Here today, gone tomorrow...and back again? A review of herbal marijuana alternatives (K2, Spice), synthetic cathinones (bath salts), kratom, Salvia divinorum, methoxetamine, and piperazines. J Med Toxicol. 2012;8(1):15-32. doi: 10.1007/s13181-011-0202-2. [PubMed: 22271566]. [PubMed Central: PMC3550220].
  • 5. Nkomo MM, Katerere DD, Vismer HH, Cruz TT, Balayssac SS, Malet-Martino MM, et al. Fusarium inhibition by wild populations of the medicinal plant Salvia africana-lutea L. linked to metabolomic profiling. BMC Complement Altern Med. 2014;14:99. doi: 10.1186/1472-6882-14-99. [PubMed: 24621275]. [PubMed Central: PMC4101822].
  • 6. Malencic D, Gasic O, Popovic M, Boza P. Screening for antioxidant properties of Salvia reflexa hornem. Phytother Res. 2000;14(7):546-8. [PubMed: 11054848].
  • 7. Bisio A, De Mieri M, Milella L, Schito AM, Parricchi A, Russo D, et al. Antibacterial and Hypoglycemic Diterpenoids from Salvia chamaedryoides. J Nat Prod. 2017;80(2):503-14. doi: 10.1021/acs.jnatprod.6b01053. [PubMed: 28186413].
  • 8. Zhang S, Liu Y, Shen S, Liang Z, Yang D. [Effects of elicitors on accumulation of phenolic acids and tanshinones in Salvia miltiorrhiza hairy root]. Zhongguo Zhong Yao Za Zhi. 2011;36(10):1269-74. Chinese. [PubMed: 21837963].
  • 9. Loussouarn M, Krieger-Liszkay A, Svilar L, Bily A, Birtic S, Havaux M. Carnosic Acid and Carnosol, Two Major Antioxidants of Rosemary, Act through Different Mechanisms. Plant Physiol. 2017;175(3):1381-94. doi: 10.1104/pp.17.01183. [PubMed: 28916593]. [PubMed Central: PMC5664485].
  • 10. Lopez-Bote CJ, Gray JI, Gomaa EA, Flegal CJ. Effect of dietary administration of oil extracts from rosemary and sage on lipid oxidation in broiler meat. Br Poult Sci. 1998;39(2):235-40. doi: 10.1080/00071669889187. [PubMed: 9649877].
  • 11. Bradford PA. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev. 2001;14(4):933-51. table of contents. doi: 10.1128/CMR.14.4.933-951.2001. [PubMed: 11585791]. [PubMed Central: PMC89009].
  • 12. Mills-Robertson FC, Tay SC, Duker-Eshun G, Walana W, Badu K. In vitro antimicrobial activity of ethanolic fractions of Cryptolepis sanguinolenta. Ann Clin Microbiol Antimicrob. 2012;11:16. doi: 10.1186/1476-0711-11-16. [PubMed: 22709723]. [PubMed Central: PMC3473295].
  • 13. Drew WL, Barry AL, O'Toole R, Sherris JC. Reliability of the Kirby-Bauer disc diffusion method for detecting methicillin-resistant strains of Staphylococcus aureus. Appl Microbiol. 1972;24(2):240-7. [PubMed: 5071651]. [PubMed Central: PMC380588].
  • 14. Cekovska Z, Petrovska M, Jankoska G, Panovski N, Kaftandzieva A. Isolation, identification and antimicrobial susceptibility of brucella blood culture isolates. Prilozi. 2010;31(1):117-32. [PubMed: 20703187].
  • 15. Thamlikitkul V, Lorchirachoonkul N, Tiengrim S. In vitro and in vivo activity of tebipenem against ESBL-producing E. coli. J Med Assoc Thai. 2014;97(12):1259-68. [PubMed: 25764632].
  • 16. Motamedi H, Darabpour E, Gholipour M, Seyyed Nejad SM. In vitro assay for the anti-Brucella activity of medicinal plants against tetracycline-resistant Brucella melitensis. J Zhejiang Univ Sci B. 2010;11(7):506-11. doi: 10.1631/jzus.B0900365. [PubMed: 20593515]. [PubMed Central: PMC2897020].
  • 17. Sharifi-Rad J, Mnayer D, Roointan A, Shahri F, Ayatollahi SA, Sharifi-Rad M, et al. Antibacterial activities of essential oils from Iranian medicinal plants on extended-spectrum beta-lactamase-producing Escherichia coli. Cell Mol Biol (Noisy-le-grand). 2016;62(9):75-82. [PubMed: 27650980].
  • 18. Shirazi MH, Ranjbar R, Eshraghi S, Amin G, Nouri MS, Bazzaz N. Inhibitory effects of Sage extract on the growth of enteric bacteria. Pak J Biol Sci. 2008;11(3):487-9. [PubMed: 18817180].
  • 19. Ying G, Zhang S, Hu Y, Yang M, Chen P, Wu X, et al. Antibacterial evaluation of Salvia miltiorrhizae on Escherichia coli by microcalorimetry coupled with chemometrics. AMB Express. 2017;7(1):65. doi: 10.1186/s13568-017-0359-4. [PubMed: 28303551]. [PubMed Central: PMC5355400].
  • 20. Yousefzadi M, Sonboli A, Ebrahimi SN, Hashemi SH. Antimicrobial activity of essential oil and major constituents of Salvia chloroleuca. Z Naturforsch C. 2008;63(5-6):337-40. [PubMed: 18669017].
  • 21. Pimchan T, Maensiri D, Eumkeb G. Synergy and mechanism of action of alpha-mangostin and ceftazidime against ceftazidime-resistant Acinetobacter baumannii. Lett Appl Microbiol. 2017;65(4):285-91. doi: 10.1111/lam.12789. [PubMed: 28799218].
  • 22. Kadry AA. Lack of efflux mechanism in a clinical isolate of Pseudomonas aeruginosa highly resistant to beta-lactams and imipenem. Folia Microbiol (Praha). 2003;48(4):529-33. [PubMed: 14533486].
  • 23. Molina-Quiroz RC, Loyola DE, Munoz-Villagran CM, Quatrini R, Vasquez CC, Perez-Donoso JM. DNA, cell wall and general oxidative damage underlie the tellurite/cefotaxime synergistic effect in Escherichia coli. PLoS One. 2013;8(11). e79499. doi: 10.1371/journal.pone.0079499. [PubMed: 24260236]. [PubMed Central: PMC3832599].
  • 24. Shigi Y, Matsumoto Y, Kaizu M, Fujishita Y, Kojo H. Mechanism of action of the new orally active cephalosporin FK027. J Antibiot (Tokyo). 1984;37(7):790-6. [PubMed: 6381449].
  • 25. Denisuik AJ, Karlowsky JA, Denisuik T, Nichols WW, Keating TA, Adam HJ, et al. In vitro activity of ceftazidime-avibactam against 338 molecularly characterized gentamicin-nonsusceptible Gram-negative clinical isolates obtained from patients in Canadian hospitals. Antimicrob Agents Chemother. 2015;59(6):3623-6. doi: 10.1128/AAC.00364-15. [PubMed: 25824208]. [PubMed Central: PMC4432173].
  • 26. Li JR, Wang YX, Wang X, Yuan B, Fu ML. Intercalation and adsorption of ciprofloxacin by layered chalcogenides and kinetics study. J Colloid Interface Sci. 2015;453:69-78. doi: 10.1016/j.jcis.2015.03.067. [PubMed: 25965434].
  • 27. Neela V, Rankouhi SZ, van Belkum A, Goering RV, Awang R. Stenotrophomonas maltophilia in Malaysia: molecular epidemiology and trimethoprim-sulfamethoxazole resistance. Int J Infect Dis. 2012;16(8):e603-7. doi: 10.1016/j.ijid.2012.04.004. [PubMed: 22698885].
  • 28. Nascimento GGF, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz J Microbiol. 2000;31(4). doi: 10.1590/s1517-83822000000400003.
  • 29. Kamatou GPP, Viljoen AM, van Vuuren SF, van Zyl RL. In vitro evidence of antimicrobial synergy between Salvia chamelaeagnea and Leonotis leonurus. South Afr J Bot. 2006;72(4):634-6. doi: 10.1016/j.sajb.2006.03.011.

Featured Image:

Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments