Review on the Laboratory diagnosis of Q-Fever

AUTHORS

Raziyeh Zabihi 1 , Keyvan Majidzadeh 2 , Amir Hossein Mohseni 3 , Mohammad Soleimani 4 , *

1 Researcher, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran., Andorra

2 Assistant Professor, Tasnim Biotechnology of Research Center (TBRC), AJA University of Medical Sciences, Tehran, Iran., Andorra

3 Researcher, Tasnim Biotechnology of Research Center (TBRC), AJA University of Medical Sciences, Tehran, Iran., Andorra

4 Assistant Professor, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran., Andorra

How to Cite: Zabihi R, Majidzadeh K , Mohseni A H , Soleimani M . Review on the Laboratory diagnosis of Q-Fever, Ann Mil Health Sci Res. 2013 ; 11(4):e64999.

ARTICLE INFORMATION

Annals of Military and Health Sciences Research: 11 (4); e64999
Published Online: December 05, 2013
Article Type: Review Article
Received: July 29, 2013
Accepted: November 05, 2013

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Abstract

Background: Coxiella burnetii is the etiologicagent of a zoonotic disease which named Q-fever in humans and coxiellosis in animals. This bacterium can survive in the environment out of the specific host. Accordingly, it categorized by the CDC in bioterrorism agents ‘category B. Consequently, rapid detection of the bacteriumadministratesthe treatment of disease. Materials and Methods: A review study on the different researches on laboratory diagnosis field in the world and Iran scientific databases was conducted. This paper includes biological safety, cultivation and detection assays. Results: Detection of Coxiella burnetii can be done byclassical methods (isolation, cultivation in the appropriate cell line such as P388D1, J774, and L929), serologic tests (immunofluorescence, micro immunofluorescence, complement fixation test and ELISA) and molecular biology methods (PCR, Nested PCR and Real Time PCR). Conclusions: While have existed kinds of detection methods for this agent, costly, need to specific laboratory and time consuming are the limitation of the mentioned techniques. Molecular methods due to accuracy and high rapidity in detection can be effective.

Keywords

Q fever Coxiella burnetii Clinical Laboratory Techniques

© 2013, Annals of Military and Health Sciences Research. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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