Development of Agglutination Test for Detection of isolated Mannoprotein Antigen from Candida Albicans

AUTHORS

Masoumeh Rajabibazl 1 , Mohammad Javad Rasaee 2 , Mahtab Nouri Fard 3 , Zohreh Farahnezhad 4 , *

1 Assistant Professor, Clinical Biochemistry Department, Faculty of Medicine, Shahid Beheshti University of Medicine, Tehran, Iran, Andorra

2 Professor, Medical Biotechnology Department, Medical Sconces Faculty, Tarbiat Modares University, Tehran, Iran, Andorra

3 - Assistant Professor, Infectious Disease Department, Faculty of Medicine, AJA University of Medical Sconces, Tehran, Iran, Andorra

4 Assistant Professor, Mycology Department, Faculty of Medicine, AJA University of Medical Sconces, Tehran, Iran, Andorra

How to Cite: Rajabibazl M , Rasaee M J, Nouri Fard M , Farahnezhad Z. Development of Agglutination Test for Detection of isolated Mannoprotein Antigen from Candida Albicans, Ann Mil Health Sci Res. 2013 ; 11(2):e65198.

ARTICLE INFORMATION

Annals of Military and Health Sciences Research: 11 (2); e65198
Published Online: May 11, 2013
Article Type: Original Article
Received: December 26, 2012
Accepted: April 11, 2013

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Abstract

Background: Candida albicans is one of the most common causes of hospital infections. The aim of this study was to develop a rapid, easy and sensitive way to detect candida albicans. Material and Methods: Polyclonal antibodies were prepared in rabbits. The antibodies were purified by salt concentration and Ion exchange chromatography. The purified antibody was coated onto the colloidal gold. Results: Color change (red to blue) was observed when the purified antigens (mannoprotein of Candida albicans cell wall) were added to Polyclonal antibodies. The method was sensitive and easy. Conclusion: This study indicated that using colloidal gold particle agglutination method can be used for accurate and rapid candida detection Method.

Keywords

Candida Albicans Mannoproteins Antigen Agglutination Gold

© 2013, Annals of Military and Health Sciences Research. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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