Application of L-Arginine as nitric oxide inducer and Indomethacin as prostaglandin inhibitor in Balb/c mice infected with Leishmania major MRHO/IR/75/ER

AUTHORS

Fatemeh Ghasemi 1 , * , Hossein Nahrevanian 2

1 Researcher, AJA University of Medical Science, Department of Biochemistry, Tehran, Iran, Andorra

2 Resercher, Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran, Andorra

How to Cite: Ghasemi F, Nahrevanian H. Application of L-Arginine as nitric oxide inducer and Indomethacin as prostaglandin inhibitor in Balb/c mice infected with Leishmania major MRHO/IR/75/ER, Ann Mil Health Sci Res. 2013 ; 11(1):e66701.

ARTICLE INFORMATION

Annals of Military and Health Sciences Research: 11 (1); e66701
Published Online: January 05, 2013
Article Type: Original Article
Received: September 25, 2012
Accepted: December 05, 2012

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Abstract

Background: Leishmaniasis is one of the health problems in. This study is designed to inhibit PG production by Indomethacin and induce NO by L- Arginine precursor in L. major infected Balb/c mice.

Materials and methods: This was an experimental study. Animals, Male inbred Balb/c mice were used in this study. The total number of animals used in this experiment was 48 Balb/c mice, divided into 6 groups (n =8 mice/group) including Group 1 (naive), Group 2 (L. major + 0.4% Ethanol), Group 3 (L. major + Indomethacin), Group 4 (L. major + DW), Group 5 (L. major + L- Arginine) and Group 6 (L. major + Indomethacin + LArginine). In addition to serum, liver and spleen suspensions were investigated for NO induction by using Griess microassay. The data was analyzed by Analysis of Variances (ANOVA) and Student’s t-test using Graph Pad Prism Software.

Results: The results indicated that production of NO was inhibited in infected Balb/c mice by L. major as compared with naive animals (Group 1). INDO as inhibitor PG (Group 2) showed their ability to elevate RNI levels in infect animals. INDO showed anti-leishmanial activity, as these compounds reduced the lesion sizes (P<0.001). INDO as inhibitor PG (Group 3) showed ability to decrease PG levels in infect animals.

Conclusion: Our data may indicate a possible role for L-Arg and INDO as novel drug targets for the treatment of cutaneous leishmaniasis in mouse model.

Keywords

Leishmania major Nitric Oxide Indomethacin L-Arginine

© 2013, Annals of Military and Health Sciences Research. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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